The Role of CD8+TLymphocytes of the Decidual Tissue in the Genesis of Early Miscarriage

Introduction. Despite the achievements of the modern obstetric and gynecological service, the
most common cause of pregnancy complications in the 1st trimester is its premature termination.
According to the World Health Organization, miscarriage rates range from 10 to 20% of all clinically
diagnosed pregnancies. Many authors noted that an important role in the successful course of
pregnancy belongs to the decidual endometrial tissue, which is involved not only in providing
nutrition to the embryo, but also in preventing immunological rejection. In the decidua during
pregnancy there is a concentration of CD8+T-lymphocytes, which are an important part of
local immune responses. Elucidating the mechanisms by which CD8+T-lymphocytes regulate
the competing processes of fetal tolerance and defense against pathogen invasion is critical
to understanding early pregnancy. The nature of the activity of CD8+T-lymphocytes and their
production of cytokines in the decidua determines the success of the invasion of trophoblast cells
and the subsequent course of pregnancy.
Purpose. Assessment of the role of CD8+T-lymphocytes of decidual tissue in the development of
early miscarriage.
Materials and methods. A clinical and morphological analysis of 102 cases of miscarriage identifi ed
in women in the Grodno region was carried out. An immunohistochemical study was performed
using primary antibodies to CD8 receptors at a dilution of 1: 100 (clone C8/144B, Dako). Marker
expression was quantifi ed using the Aperio Image Scope_v9.1.19.1567 computer program.
Results. The level of CD8 expression in the decidual tissue of patients with miscarriage is signifi cantly
lower than during physiological pregnancy (p<0.00008). Conclusion. If the level of positivity of CD8 expression in decidual tissue is less than or equal to 0.173, it is possible to predict miscarriage with a sensitivity of 100% and a specifi city of 95.8%.